|
KMID : 1098419960040020132
|
|
Korean Journal of Medicinal Crop Science
1996 Volume.4 No. 2 p.132 ~ p.138
|
|
|
The Effect of Culture Methods and Plant Growth Regulators on Bulblet Formation and Growth in Scale Segment Culture of Fritillaria Thunbergii Miq
|
|
유광진/Yu, Kwang Jin
백기엽/Pa다, Kee Yoeup
|
|
|
Abstract
|
|
|
|
This experiment was carried out to establish a year-round production system of pathogen-free stock through micropropagation in Fritillaria thunbergii as medicinal bulbous plant. The effect of different types of culture method and plant growth regulators, activated charcoal and mannitol on bulblet formation and subsequent growth were investigated. The MS solid medium containing 1. 0 mg/L kinetin and 0. 3 mg/L NAA was effective on bulblet formation and propagation rate compared to liquid and suspension culture. Addition of activated charcoal at 0. 01% to 0. 1% in the medium promoted bulbing of cultured bulblets and shoot formation. Addition of 1% to 2% mainnitol in MS medium was effective on the formation of bulblet and subsequent growth of bulblets compared to control. In addition of inhibitors, $10{\sim}100\;mg/L$ 수식 이미지 B-9 and Chloromequat had effective to stimulate bulblet growth but their effects were not so much as mannitol treatment. ABA treatment had detrimental effect on survival rate of explant and bulblet formation.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|